Preparing a cyanobacterial chassis for H2 production: a synthetic biology approach
Institute for Molecular and Cell Biology (IBMC) –
Molecular hydrogen (H2) is an environmentally clean energy carrier that can be a valuable alternative to the limited fossil fuel resources of today. The BioModularH2 project aims at designing reusable, standardized molecular building blocks that integrated into a “chassis” will result in a photosynthetic bacterium containing engineered chemical pathways for competitive, clean and sustainable hydrogen production. For this project the unicellular cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis) is being used as the photoautotrophic “chassis” for this project.
To prepare the chassis for an optimal H2 production, the Synechocystis native bidirectional hydrogenase was inactivated. Later on, a synthetic circuit containing a heterologous highly efficient hydrogenase will be introduced into the “chassis”. Due to hydrogenase sensitivity to molecular oxygen, and to provide the anaerobic environment required for an optimal heterologous hydrogenase activity, synthetic oxygen consuming devices are being prepared based on native and heterologous enzymes that use O2 as substrate, and will be subsequently tested.
Finally, the integration of the designed synthetic circuits into the “chassis” will provide an anaerobic environment within the cell for an optimized and highly active hydrogenase.
Date: 2009-Oct-09 Time: 14:00:00 Room: 336
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Workshop “Metabolism and mathematical models: Two for a tango” – 2nd Edition
Title: Workshop Metabolism and mathematical models: Two for a tango – 2nd Edition
Dates: October 25-26, 2022
Location: This workshop will be held in a virtual way
The topic of this workshop is metabolism in general, with a special focus, although not exclusive, on parasitology. Besides an exploration of the biological, biochemical and biomedical aspects, the workshop will also aim at presenting some of the mathematical modelling, algorithmic theory and software development that have become crucial to explore such aspects.
This workshop is being organised in the context of two projects, both with the Inria European Team Erable. One of the projects involves a partnership with the University of São Paulo (USP), in São Paulo, Brazil, more specifically the Institute of Mathematics and Statistics (IME) and the Institute of Biomedical Sciences – Inria Associated Team Capoeira – and the other involves the Inesc-ID/IST in Portugal, ETH in Zürich and EMBL in Heidelberg – H2020 Twinning Project Olissipo.
The workshop is open to all members of these two projects but also, importantly, to the community in general.
The program and more details are available here.